Luhaibi, Maadh Jumaah Owaid (2025) Synthesis And Characterisation Of New Fluorescent Pyrazoline- Piperazinyl Linked Paclitaxel As Prodrugs: Photodynamic Therapy And Anticancer Activity Against Mcf-7 And Mcf-10a Cell Lines. PhD thesis, Perpustakaan Hamzah Sendut.
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Abstract
This study involves the synthesis of new pendarflour compounds with combination of photodynamic therapy (pdt) and chemotherapy activity against mcf-7 (cancer cells) and mcf-10a (normal cells) which is an effective strategy to treat cancers. A unique photosensitizer (1-17)a (ps)-conjugated paclitaxel (ptx) (1-15)c prodrug has been developed, in which a ps is excited by near-infrared wavelength light to specifically release ptx while generating singlet oxygen (so) to kill cancer cells. The mechanism involves the use of singlet oxygen to cleave aminoacrylate-type linkers in the prodrug. This generates reactive oxygen species (ros) that induce oxidative damage and cell death. Mcf-7 cell death was triggered by ros surpassing the cell’s redox capacity, leading to apoptosis, confirmed by caspase glo-3/7 assays. A paclitaxel (ptx)-based photosensitive conjugate was developed, where light exposure releases intact ptx, combining chemotherapy with immediate pdt action. Pyrazoline (ps) (1-17)a, a key component of the conjugate, was found to be non-toxic and effective in pdt. Uv/vis absorption and fluorescence emission characteristics were evaluated, revealing the stability and reactivity of the photosensitizer. In vitro studies demonstrated that py-l-ptx induced cell death in the presence of light without affecting cells in the dark.
| Item Type: | Thesis (PhD) |
|---|---|
| Subjects: | Q Science > QD Chemistry > QD1-999 Chemistry |
| Divisions: | Pusat Pengajian Sains Kimia (School of Chemical Sciences) > Thesis |
| Depositing User: | Mr Hasmizar Mansor |
| Date Deposited: | 19 Jun 2026 01:06 |
| Last Modified: | 19 Jun 2026 01:06 |
| URI: | http://eprints.usm.my/id/eprint/64404 |
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