Extraction, Characterisation And Bioactivity Assessment Of Pectic-Polysaccharide From Cinnamomum Verum Using Deep Eutectic Solvent–Microwave Assisted Approach

Beh, Chi Kin (2025) Extraction, Characterisation And Bioactivity Assessment Of Pectic-Polysaccharide From Cinnamomum Verum Using Deep Eutectic Solvent–Microwave Assisted Approach. PhD thesis, Universiti Sains Malaysia.

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Abstract

The cosmetics market exhibited increasing demand in natural-based product with excellent anti-hyperpigmentation effects. Meanwhile, the research community is venturing on extraction solvents that is eco-sustainable. Despite increasing interest, a Deep Eutectic Solvent (DES) specifically tailored for polysaccharide extraction remained undeveloped. In response, a novel acidic DES was synthesized from cost-effective, readily available components: citric acid monohydrate and L-glutamic acid in a 1:1 molar ratio, using microwave-assisted heating (10 min, 360 W). The resulting DES exhibited an orange hue, attributed to the involvement of the Maillard reaction during synthesis, indicating a synergistic interaction between the parent compounds. Characterization revealed that the DES is hydrophilic and acidic, with full water miscibility. Physicochemical evaluations included pH, density, solubility, rheological behaviour, and FTIR spectroscopy, alongside Maillard reaction assessments. Molecular docking analysis further supported the presence of a robust hydrogen-bond network between the amine and carboxyl groups of glutamic acid, carboxyl groups of citric acid, and water molecules, stabilizing the supramolecular structure. This newly synthesized DES demonstrated strong potential for both laboratory-based research and scale-up for polysaccharide extraction

Item Type: Thesis (PhD)
Subjects: Q Science > QD Chemistry > QD415-436 Biochemistry
Divisions: Pusat Penyelidikan Biokimia Analisis (Analytical Biochemistry Research Centre (ABrC)) > Thesis
Depositing User: Mr Aizat Asmawi Abdul Rahim
Date Deposited: 09 Jun 2026 07:37
Last Modified: 09 Jun 2026 07:37
URI: http://eprints.usm.my/id/eprint/64342

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