Yiadom, Osei George (2025) Analysis Of The Effects Of Microrna-130a Suppression On The Proliferation And Characteristics Of Cancer Stem Cells In The Human Colorectal Cancer Cell Lines. Masters thesis, Perpustakaan Hamzah Sendut.
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Abstract
Colorectal cancer (crc) is a significant global health burden. Growing evidence highlights the role of cancer stem cells (cscs) in mediating treatment resistance and disease recurrence. Micrornas (mirnas) have emerged as crucial regulators in cancer biology, with mir-130a demonstrating a multifaceted impact. This study therefore explores the expression and functional significance of mir-130a-3p (herein referred to as mir-130a) within crc, with a particular emphasis on its potential association with cscs. Reverse transcription quantitative real-time pcr (rt-qpcr) analysis revealed significantly higher mir-130a levels in crc cell lines (ht-29, hct 116, and caco-2) compared to normal colon epithelial cells (ncm460). This finding was corroborated by the cancer genome atlas (tcga) data, showing mir-130a overexpression in colon and rectal adenocarcinoma tissues compared to healthy controls. Tcga data analysis also indicated weak inverse correlations between mir-130a expression and established stemness markers (epcam, bmi1, cd44, and prom1), suggesting a possible suppressive effect of mir-130a on cscs. To determine mir-130a's functional role, its expression was suppressed in ht-29 cells using lenticrisprv2 plasmids.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | R Medicine > R Medicine (General) > R5-920 Medicine (General) |
| Divisions: | Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute (AMDI)) > Thesis |
| Depositing User: | Mr Hasmizar Mansor |
| Date Deposited: | 08 Jun 2026 06:39 |
| Last Modified: | 08 Jun 2026 06:39 |
| URI: | http://eprints.usm.my/id/eprint/64303 |
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