Application Of In Silico And In Vitro Approaches On Repurposed Drugs Targeting Putative Rho Gtpase And Rhogap Of Giardia Lamblia.

Jasni, Nurhana (2025) Application Of In Silico And In Vitro Approaches On Repurposed Drugs Targeting Putative Rho Gtpase And Rhogap Of Giardia Lamblia. Masters thesis, Perpustakaan Hamzah Sendut.

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Abstract

Giardia lamblia is a globally distributed protozoan parasite that causes an intestinal disease named giardiasis. The primary treatment relies on nitroimidazole drugs such as metronidazole, tinidazole, and albendazole. However, the incidence of refractory cases had increased, leading to therapeutic non-compliance. Although numerous research studies have been conducted to address these concerns, they remain unresolved. The small gtpase, rho subfamily protein, and its positive regulator, putative rho gtpase activating protein (rhogap), were found to be involved in various biological and cellular processes. They were found to work in tandem in regulating encystation and membrane trafficking and were involved in the metabolic processes of this protozoan parasite. Thus, it was hypothesized that inhibiting these proteins would lead to the killing of the parasite. The target sequence analysis showed that two repurposed drugs, dextromethorphan and azathioprine, were matched to the small gtpase, rho subfamily proteins. On the other hand, three re-purposed drugs were matched to the putative rhogap, which were imatinib, dasatinib, and ponatinib. Molecular docking analysis showed the binding energies of the five drugs to their respective proteins were -8.5 kcal/mol, -8.0 kcal/mol, -7.0 kcal/mol, -5.3 kcal/mol, and -6.8 kcal/mol, represented by imatinib, ponatinib, dasatinib, azathioprine, and dextromethorphan, respectively. Molecular

Item Type:	Thesis (Masters)
Subjects:	R Medicine > R Medicine (General) > R5-920 Medicine (General)
Divisions:	Institut Penyelidikan Perubatan Molekul (Institute for Research in Molecular Medicine INFORMM) > Thesis
Depositing User:	Mr Hasmizar Mansor
Date Deposited:	04 Jun 2026 06:58
Last Modified:	04 Jun 2026 06:58
URI:	http://eprints.usm.my/id/eprint/64290

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