Thangeswaran, Danesh (2025) Modulatory Effect Of Thicapa On The Amyloid Precursor Protein Processing Pathways Of Familial Alzheimer’S Disease Using Fibroblast Cell Lines. Masters thesis, Perpustakaan Hamzah Sendut.
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Abstract
Familial alzheimer’s disease (fad) is a hereditary and irreversible neurological disorder characterised by the accumulation of toxic amyloid-beta (aβ). The prevalence of fad symptoms is concerning as there are no available therapeutic solutions to cure this disease. Thicapa is a novel compound belonging to the tetrahydroisoquinoline (thiq) group of amines, which reported the neuroprotective effects on the ad transgenic drosophila melanogaster (d. Melanogaster) model. Therefore, this study investigated the modulatory effect of thicapa on the amyloid precursor protein (app) processing pathway using the fad patient skin-derived fibroblast cell line (ag06840). This study utilized wst-1 assay to assess cytotoxicity and scavenging potency of thicapa, while qrt-pcr and western blot to analyse gene and protein expressions, respectively. Additionally, protein quantification was performed using elisa and intracellular reactive oxygen species (ros) levels were measured using the dcfda assay. The cytotoxicity assay recorded the insignificant toxic effect of thicapa towards ag06840 and healthy skin-derived fibroblast cell line (gm05879) at three different time points of 24, 48, and 72 hours. The subsequent aβ scavenging assay showed that 50 μm thicapa exerted a potent scavenging effect on aged aβ42 oligomers in the gm05879 fibroblasts cell line.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | R Medicine > R Medicine (General) > R5-920 Medicine (General) |
| Divisions: | Institut Penyelidikan Perubatan Molekul (Institute for Research in Molecular Medicine INFORMM) > Thesis |
| Depositing User: | Mr Hasmizar Mansor |
| Date Deposited: | 20 May 2026 00:57 |
| Last Modified: | 20 May 2026 00:57 |
| URI: | http://eprints.usm.my/id/eprint/64246 |
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