The Effects Of Physical Stressors And Neurotransmitters On The Spawning Activity Of The Sea Cucumber, Stichopus Fusiformiossa

Cheong, Kah Mei (2025) The Effects Of Physical Stressors And Neurotransmitters On The Spawning Activity Of The Sea Cucumber, Stichopus Fusiformiossa. Masters thesis, Perpustakaan Hamzah Sendut.

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Abstract

Stichopus fusiformiossa a sea cucumber species from the stichopodidae family, known as gamat have been integral to malaysian traditional medicine and is now increasingly valued in pharmaceuticals, nutraceuticals, cosmetics, and food products. However, excessive harvesting and insufficient conservation measures have endangered this sea cucumber species. To address this issue, enhancing breeding methods, specifically artificial spawning, is essential for sustainable aquaculture. Therefore, this research aims to develop a spawning protocol for s. Fusiformiossa by investigating the potential of neurotransmitters to regulate gamete release and fertilisation. This research represents the first attempt to induce spawning in s. Fusiformiossa using physical methods: heat shock, cold shock, desiccation, and microalgae stimulation and neurotransmitter injections: serotonin (5-ht), 1-methyladenine (1-ma), epinephrine, and norepinephrine in captivity. The sea cucumbers were observed for eight hours post-induction. Histological analysis of the gonads was conducted randomly and provided insights into reproductive development stages. An in-vitro germinal vesicle breakdown (gvbd) assay was conducted on s. Fusiformiossa oocytes, using 5-ht and 1-ma at two concentrations (1×10-1 m and 1×10-3 m) over three incubation durations (60, 120, and 180 minutes).

Item Type: Thesis (Masters)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Pusat Kajian Samudera & Pantai (Centre for Marine and Coastal studies (CEMACS)) > Thesis
Depositing User: Mr Hasmizar Mansor
Date Deposited: 20 May 2026 00:56
Last Modified: 20 May 2026 00:56
URI: http://eprints.usm.my/id/eprint/64245

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