Evaluation Of Polyhydroxyalkanoate (Pha) Films Containing Stingless Bees (Geniotrigona Thoracica) Propolis For Burn Wound Healing In A Streptozotocin-Induced Diabetic Rat Model

Salih, Samar Abdelrazeg Abdelrahman (2025) Evaluation Of Polyhydroxyalkanoate (Pha) Films Containing Stingless Bees (Geniotrigona Thoracica) Propolis For Burn Wound Healing In A Streptozotocin-Induced Diabetic Rat Model. PhD thesis, Perpustakaan Hamzah Sendut.

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Abstract

Wounded or damaged tissues on the skin are replaced with freshly created new tissues during the wound healing process. Propolis exhibit diverse array of bioactivities and chemical composition which possess nutritive, medicinal and health benefits, including the potential as a wound healing agent. Pha is a synthetic biocompatible polymer material which has potentials for a wide range of medical applications. In this study, the properties and characteristics of pha and propolis were studied to provide a novel, low-cost, effective, and safe wound dressing patches. This polymer-based film containing propolis is a novel strategy for treatment of skin wounds. Propolis was extracted and optimised in vitro using the human dermal fibroblasts (hdf) cells to demonstrate biocompatibility, cellular migration and angiogenic gene expression. Hdf cells viability was tested to determine the least toxicity of dmso concentration in the propolis media. Pha was synthesized from c. Necator, characterised and tested for its biocompatibility. Stz at an optimised dose of 65mg/kg had successfully induced diabetes in the rats as confirmed by blood sugar analysis. Patches were fabricated and propolis was loaded to the patch just before applying it to the wound created by using a self-made heated rod. Concentration of 0.1% (v/v) dmso gave the highest hdf viability. In a serial dilution optimisation step, the concentration of 10 μg/ml of propolis gave the highest cell viability (100%).

Item Type: Thesis (PhD)
Subjects: R Medicine > R Medicine (General) > R5-920 Medicine (General)
Divisions: Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute (AMDI)) > Thesis
Depositing User: Mr Hasmizar Mansor
Date Deposited: 15 May 2026 06:58
Last Modified: 15 May 2026 06:58
URI: http://eprints.usm.my/id/eprint/64212

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