Ibrahim, Muhammad (2025) In Vitro And In Silico Analysis Of Sphingobacterium Sp. Lipase Co-Expression With Lea K Peptide. PhD thesis, Perpustakaan Hamzah Sendut.
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Abstract
A yellowish gram-negative bacterium called sphingobacterium sp. Is frequently distinguished by large quantities of sphingophospholipids in its lipid composition. Since few studies have been done on the sphingobacterium sp. Lipase, which has been the subject of limited research, this study aims to provide essential knowledge on the lipase activities of sphingobacterium sp. With lea-k as co-expression and in silico studies. To characterize ab3 lipase from sphingobacterium sp. That has been produce with lea k. To predict the 3-dimensional structure of sphingobacterium sp. Lipase and lea-k peptide using an in-silico approach. To investigate the interaction of ab3 lipase-lea k peptide complex that enhanced catalytic activity using in silico studies. Therefore, this study's results will be more helpful in providing a basic grasp of favourable application values for sphingobacterium sp. Lipase. Olive oil was used as the substrate in a colourimetric test technique to measure lipase activity. The lipase structure was predicted using the swiss-model website, and molecular docking was done using the autodock vina program to investigate molecular interactions. The optimum expression conditions were determined at 25ºc for 24 hours of incubation with 0.5mm iptg.
| Item Type: | Thesis (PhD) |
|---|---|
| Subjects: | Q Science > QH Natural history > QH1-278.5 Natural history (General) |
| Divisions: | Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis |
| Depositing User: | Mr Hasmizar Mansor |
| Date Deposited: | 13 May 2026 02:31 |
| Last Modified: | 13 May 2026 02:31 |
| URI: | http://eprints.usm.my/id/eprint/64179 |
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