Gold Nanoparticle-induced Chondrogenic Differentiation Of Human Umbilical Cord Blood Mesenchymal STEM Cells (Hucb-msc) For Osteoarthritis Therapy

Suhimi, Anis Athiqah (2023) Gold Nanoparticle-induced Chondrogenic Differentiation Of Human Umbilical Cord Blood Mesenchymal STEM Cells (Hucb-msc) For Osteoarthritis Therapy. Masters thesis, Universiti Sains Malaysia.

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Abstract

The pressing need for new solutions in osteoarthritis therapy is highlighted by its global impact on millions, healthcare resources, and daily activities, alongside the difficulty of cartilage healing and limitations of age-related bone marrow derived MSCs. This research highlights the significant potential of using human Umbilical Cord Blood Mesenchymal Stem Cells (hUCB-MSCs in regenerating damaged skeletal tissues, particularly in the context of osteoarthritis. The research objectives encompass the characterization of the surface morphology of immobilized AuNPs using Scanning Electron Microscopy, analysis of hUCB-MSC viability and proliferation following treatment with various types of functionalised AuNPs using Trypan Blue exclusion assay and RT-PCR and the investigation of hUCB-MSC differentiation in the presence of AuNP treatments using immunofluorescence staining and MSC functional identification assay. The research delves into the role of hydrophobic and hydrophilic AuNPs, and influence of AuNP intemalisation which may impact hUCB-MSC, and effects of AuNP spacing towards focal adhesion and cell differentiation. Characterisation of immobilised gold nanoparticles was able to be achieved by using scanning electron microscopy technique paired with ImageJ software analysis. Due to unsuccessful attempts of cell culture, further research objectives were not able to be performed.

Item Type: Thesis (Masters)
Subjects: R Medicine > R Medicine (General)
Divisions: Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute (AMDI)) > Thesis
Depositing User: MUHAMAD AMIN BIN AZMI
Date Deposited: 13 May 2026 01:56
Last Modified: 13 May 2026 01:56
URI: http://eprints.usm.my/id/eprint/64171

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