Lee, Zun Yip (2025) Establishment Of Tissue Culture Planting Materials Of Mango (Mangifera Indica L.) Cv. Harumanis. Masters thesis, Perpustakaan Hamzah Sendut.
![[img]](http://eprints.usm.my/style/images/fileicons/application_pdf.png) |
PDF
Download (342kB) |
Abstract
Harumanis mango is one of the highest demand mango cultivars in malaysia due to its exceptional sweetness and fabulous fragrance. It is grown in a northern state of malaysia, perlis. However, the production of this mango cannot meet the market demand as there are several limitations in its propagation. The limited grafting activity and the limited number of seeds (only one harvest season per year) make vegetative and non-vegetative propagation of harumanis time and labour intensive. Micropropagation using tissue culture techniques is a reliable and effective alternative for mass in vitro propagation of harumanis at a consistent and faster rate, producing clones of the mother plants. Few studies have used nodal segments as starting material and the reported success rate is low. This is due to the two main problems, namely deep-seated contaminants and the recalcitrant nature of the plant. The objective of the present study is to develop an effective disinfection and shoot proliferation protocol for mangifera indica cv. Harumanis. A sterility rate of 87.5% was achieved by pretreating mother plants with 0.5 ml/l azoxystrobin two days before the experiment and surface sterilising nodal segments at the immature green leaf stage with 40% clorox® for 20 minutes, 0.5 g/l benomyl for 1 hour and 300 mg/l cefotaxime for 5 minutes. The transverse thin layer (ttcl) technique was applied to the nodal segments and cultures were maintained at wpm with 0.5 g/l benomyl and 300 mg/l cefotaxime.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | Q Science > QH Natural history > QH1-278.5 Natural history (General) |
| Divisions: | Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis |
| Depositing User: | Mr Hasmizar Mansor |
| Date Deposited: | 12 May 2026 01:08 |
| Last Modified: | 12 May 2026 01:08 |
| URI: | http://eprints.usm.my/id/eprint/64155 |
Actions (login required)
 |
View Item |
