Determination of total microorganisms count and vibrio species in crustaceans

Lutfi, Nurul Allia Ezzati Md (2017) Determination of total microorganisms count and vibrio species in crustaceans. Project Report. Universiti Sains Malaysia. (Submitted)

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Abstract

Vibrio microorganisms are very common in coastal environments, thus they are usually present in seafood. The most prevalent Vibrio microorganisms discovered in seafood, particularly crustaceans are V parahaemolyticus, V cholerae, and V vulnificus. However, currently in Malaysia, there is no set standard for the permissible limit of Vibrio microorganisms in crustaceans. Hence, the aim of this study was to determine their presence in crustaceans available in Malaysia. The methodology being utilized in this study was as according to Bacteriological Analytical Methods from the Food and Drug Administration (FDA). For this study, 10 different species of crustaceans were purchased from markets around Kota Bharu, Kelantan and were analyzed both for the total microorganisms count and Vibrio microorganism's profile. The results had shown that Metapemeopsisbarbata, or sand prawn was the type of crustaceans which had the highest microorganisms count at 1.33x 1010cfu/g as compared to the other tested crustaceans. 30% of the samples tested had a higher microorganisms count than the established standard, in which that the limit for total microorganisms count in fishery products stated by Ministry of Health Malaysia is at most at 106cfu/g. This may posed a threat to those who are unaware of the risk that may be present. Around 60% of the crustaceans harboured both V parahaemolyticusand V cholerae, which could lead to health complications if prepared inappropriately, particularly to those with compromised immune system. In conclusion, by making this information accessible to the general population, the incidence of food poisoning due to Vibrio microorganisms could be decreased and proper handling of crustaceans during preparation could be widely practiced.

Item Type: Monograph (Project Report)
Uncontrolled Keywords: microorganisms
Subjects: R Medicine
R Medicine > RA Public aspects of medicine
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Kesihatan (School of Health Sciences) > Monograph
Depositing User: Mr Abdul Hadi Mohammad
Date Deposited: 22 Jun 2026 07:38
Last Modified: 22 Jun 2026 07:38
URI: http://eprints.usm.my/id/eprint/63867

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