Alixsius, Fatin Nursyafiqah (2025) Near-infrared glucose sensing in adulterated honey using smartphone-based colorimetric detection. Other thesis, Universiti Sains Malaysia.
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Abstract
Honey adulteration particularly with glucose raises serious health and economic problems, especially in areas where honey is highly valued for its health values. Nuclear magnetic resonance (NMR), High-Resolution Mass Spectrometry (HRMS) and Isotope Ratio Mass Spectrometry (IRMS) are the examples of laboratory based analytical technique applied to differentiate between pure and adulterated honey. However, they are not publicly accessible. This study explores a quick, affordable, and easy to-use method for measuring the concentration of glucose in adulterated honey. Sample solutions with different glucose concentrations were prepared and exposed with near-infrared (NIR) LED with 940nm wavelength. Then the image is captured and analysed using ImageJ. The mean red-green-blue (RGB) values for each sample are recorded. After that, absorbance and transmittance values are calculated using the Beer Lambert Law formula. The obtained findings for R2 value of the glucose-absorbance value are 0.5562, 0.5435 and 0.5414 respectively for red, green and blue component. This shows a strong higher correlation between the absorbance value and glucose concentration that indicates strong sensitivity. In addition, there is significant difference can be observed between RGB mean pixel intensity in category of pure honey and added pure honey with 50% water and glucose (100 g/dL). Therefore, this study can be used to differentiate between adulterated and pure honey. This illustrates that this study has potential as a useful tool for on-site honey authenticity screening, improving consumer accessibility and helping in combating against honey adulteration.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Near-infrared glucose sensing |
| Subjects: | R Medicine R Medicine > RC Internal medicine > RC71-78.7 Examination. Diagnosis Including raiography |
| Divisions: | Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Thesis |
| Depositing User: | MUHAMMAD AKIF AIMAN AB SHUKOR |
| Date Deposited: | 15 Oct 2025 06:43 |
| Last Modified: | 04 Nov 2025 08:00 |
| URI: | http://eprints.usm.my/id/eprint/62981 |
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