Synthesis And Characterisation Of Pegylated Gold Nanoparticles For Enhancement Of Agglutination Reaction In Antibody Screening Test

Ishak, Nordiyana (2024) Synthesis And Characterisation Of Pegylated Gold Nanoparticles For Enhancement Of Agglutination Reaction In Antibody Screening Test. Masters thesis, Universiti Sains Malaysia.

[img]
Preview
PDF
Download (549kB) | Preview

Abstract

Red blood cell (RBC) antigen-antibody agglutination is a crucial reaction in antibody screening tests (AST) to identify the presence of unexpected clinically significant antibodies in the plasma recipient. However, with the current method using low-ionic strength solution (LISS) as an enhancement medium for detecting antibody in AST, several drawbacks such as the missing detection of some clinically significant antibodies, and thus, can cause an adverse transfusion reaction. Hence, a new solution using polyethylene glycol with molecular weight of 4000 (PEG4000) conjugated with gold nanoparticles (AuNPs) was synthesised to produce a better RBC antigen-antibody agglutination enhancement capacity in AST. The synthesised PEG-AuNPs solution was characterised for optical properties by Ultraviolet-Visible Spectroscopy (UV-Vis), morphological characterisation by Energy-Filtered Transmission Electron Microscopy (EFTEM) and Field Emission Scanning Electron Microscope (FESEM), particle size and zeta potential analysis by Dynamic Light Scattered (DLS), and structural and composition of bonding material by Fourier-transform infrared (FT-IR) spectroscopy. Then, the RBC agglutination scoring using tube method was compared between the synthesised solution with standard LISS in AST using 120 samples consisted of 60 negative and 60 positive samples of antibody. The final colour of the solution changed to ruby red indicating the successful formation of PEG-coated with AuNPs.

Item Type: Thesis (Masters)
Subjects: R Medicine > RC Internal medicine > RC254-282 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute (AMDI)) > Thesis
Depositing User: Mr Noor Azizan Abu Hashim
Date Deposited: 12 Jun 2025 08:33
Last Modified: 12 Jun 2025 08:33
URI: http://eprints.usm.my/id/eprint/62462

Actions (login required)

View Item View Item
Share