Manisekaran, Hemagirri
(2024)
The Antiaging Activity Of Polyalthia Longifolia (Sonn.) Thwaites Methanolic Extracts On Saccharomyces Cerevisiae By611 Yeast Cells.
PhD thesis, Perpustakaan Hamzah Sendut.
Abstract
The pursuit of natural-based antiaging agents has gained momentum in recent years. Polyalthia longifolia, a valuable indigenous medicinal plant, is widely used in traditional medicines due to its high polyphenol concentration. This study assessed the anti-aging potential of P. longifolia leaf methanolic extracts (PLME) using Saccharomyces cerevisiae strain BY611 as a model organism. In this research, an integrated approach, encompassing in vitro, in situ assays and RNA sequencing techniques was employed to investigate the anti-aging properties of PLME. The methanolic extract was prepared, and rutin quantification yielded 0.013 μg/mL (1.30%) rutin content in 1000 μg of PLME. Furthermore, an optimal concentration of 1 mg/mL PLME was determined for achieving maximum anti-aging effects. Intriguingly, PLME treatment significantly (p < 0.05) extended both replicative and chronological lifespans and total lifespan while enhancing cellular proliferative potential compared to the vehicle control group. Corroborated by light microscopy, PLME also promoted longevity in BY611 yeast cells by mitigating the progression of large apoptotic-like Type 3 vacuoles.
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