Zhang, Haoling
(2024)
Construction Of Gene Expression
Model On Relationship Between
Candida Albicans And Colorectal
Cancer.
Masters thesis, Universiti Sains Malaysia.
Abstract
This study primarily explores the paradox of whether Candida albicans (C.
albicans) promotes or inhibits the development of Colorectal cancer (CRC), focusing
on its metabolites mixture for relevant arguments. Differential gene analysis was
initially employed to construct a model of differential gene-related prognostic genes
(mRNAs) and was internally and externally validated within biological information
databases. The impact of metabolites mixture on CRC cell viability was assessed
utilizing the CCK-8 assay at different time intervals (12, 24, 48, and 72 hours) and
concentrations (OD600 approximately 0.2, 0.3, 0.4). qRT-PCR was employed to
assess the mRNA expression levels of prognostic genes EHD4, LIME1, GADD45B,
TIMP1, and FDFT1 in each group of CRC cells. Cell invasion and migration
capabilities were evaluated through Transwell experiments. Finally, the eATP content
was determined using an ATP detection kit. This study identified a total of 213
differentially expressed genes. A prognostic model containing 5 specific mRNA
markers, namely EHD4, LIME1, GADD45B, TIMP1, and FDFT1, was constructed.
C. albicans metabolites mixture reduced CRC cell activity. qRT-PCR results showed
that compared to normal colonic epithelial cells, LIME and EHD4 were
downregulated in CRC cells, while FDFT1 expression was significantly upregulated.
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