Ong, Tee Gee
(2019)
Development Of A Chronological Life
Span Assay For Ageing Studies In
Saccharotnyces Cerevisiae.
Masters thesis, Universiti Sains Malaysia.
Abstract
The Saccharomyces cerevisiae. chronological life span (CLS) is the length of
time that yeast cells survive in a non-dividing state. In the laboratory, CLS is
typically measured by its optical density at the post-diauxic phase that begins
approximately two days after initial inoculation. In this study, the development of a
CLS assay on a 96-well microplate with different yeast strains and growth
parameters (media and time) were carried out. After the evaluation of four different
strains, yeast strain BY25929 was found to be the most suitable in tenns of growth
for the CLS assay. A total of 24 ageing-related proteins were selected for assessment
in the CLS assay.
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