Mostafa, Hamza Mohamed Amin
(2015)
Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers.
Masters thesis, Perpustakaan Hamzah Sendut.
Abstract
Alcohol misuse is a ravaging public health and social problem. Alcoholdependence
(AD) is a phase of alcohol misuse in which the drinker consumes
excessive amount of alcohol and have a continuous urge to consume alcohol. The
current methods of alcohol dependence diagnosis are questionnaires and some
biomarkers. However, both methods lack specificity and sensitivity. Metabolomics is
a novel scientific field which may provide a novel method for the diagnosis of
alcohol-dependence by using a sensitive and specific technique such as nuclear
magnetic resonance (NMR). Our aim was to identify the metabolic fingerprint
(metabotype) that is able to discriminate between AD, non-AD alcohol drinkers and
controls using metabolomics approach. The reproducibility of the outcomes was also
investigated. Blood and urine samples were collected from 30 alcohol-dependent
(mean age: 45.7), 54 social drinkers (mean age: 39.5) and 60 controls (mean age:
37.1). Plasma was separated by centrifugation, then both plasma and urine samples
were mixed with phosphate buffer which was prepared by dissolving di-sodium
hydrogen phosphate (Na2HP04), sodium dihydrogen phosphate (NaH2P04), sodium
3-(trimethylsilyl)-propionate-2,2,3,3-
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