Abu Sepian, Noor Raihana
(2014)
Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116.
Masters thesis, Perpustakaan Hamzah Sendut.
Abstract
This study focuses on the biotransformation of synthetic and extracted ferulic
acid (FA) from lignocellulosic biomass using growing cells of Amyco/alopsis sp.
ATCC 39116 as a biocatalyst. Preliminary alkaline hydrolysis showed that the
hydrolyzate from oil palm empty fruit bunch (OPEFB) contained the highest amount
of FA (199.4 mg/l) among the other three lignocellulosic wastes (i.e. oil palm frond,
coconut fiber and sheIl). Optimization of extraction was carried out using Response
Surface Methodology (RSM) via Central Composite Design (CCD) on the effect of
three independent variables, i.e. concentration of NaOH (1.5-5 M), solid/liquid ratio
(0.03-0.084 g/ml) and time (16-32 h). The optimum FA concentration of 2.04 mg/g
OPEFB (365.19 mg/l) was obtained under the optimal conditions of 1.5 M ofNaOH
concentration, 0.03 g/ml of solid/liquid ratio and 32 h of extraction time. The
extracted FA was further purified using polyvinylpolypyrrolidone (PVPP) and
activated carbon, and about 97.26% was successfully recovered from the extract
using PVPP. The partially purified FA was converted into 38.47% molar yield of
vanillin, showing the ability of Amyco/alopsis sp. ATCC 39116 to convert the
extracted FA from lignocellulosic waste.
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