Noor, Nurul Shahfiza
(2014)
Diagnosis Of Dengue Infection Using Metabolome Analysis (Biomarkers Profiling).
Masters thesis, Perpustakaan Hamzah Sendut.
Abstract
Dengue is a major health and pressing threat to Malaysia with increasing atypical
manifestations. These including encephalopathy, hepatic failure, renal failure, myocarditis
and rhabdomyolysis which.have been attributed chiefly to metabolic alterations. Therefore,
the metabolomics approach was used to investigate for metabolite candidates that potentially
could be utilized as an indicator for dengue disease; leads to dengue severity. In this study,
attempt was made to determine the metabolic response associate with dengue infection by
means of 11-1 NMR-based metabolomic spectrometry. We also explored the differences in
gender infected with dengue disease. Fifty-two patients diagnosed with dengue fever at
Penang General Hospital and fourty-three healthy individuals were recruited in this study.
The mid-stream urine collected was analyzed with 11-1 NMR spectroscopy, followed by
chemometric multivariate analysis. Two types of pattern recognitions were employed,
namely, Principle Component Analysis (PCA) and Orthogonal Partial Least SquareDiscriminant
Analysis (OPLS-DA). NMR signals which highlighted in the OPLS-DA S-plot
were further selected and identified using Human Metabolome Database (HMDB), Chenornx
Profiler and from published literature.
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