Omar, Rosila (2015) Polarographic determination of ascorbic acid in roselle juice samples. Project Report. Universiti Sains Malaysia. (Submitted)
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Abstract
Ascorbic acid which is also known as vitamin C cannot be synthesised in human body. Hence, it is important to get the vitamin C from other source such as roseile juice. The recommended daily intake of vitamin C is 60 mg/day. Polarography technique has been used to determine the concentration of vitamin C in the roseile juice samples. The parameters for polarography technique for ascorbic acid determination are as follows: start potential: -0.0499 V, end potential: 0.1299 V, deposition potential: 0.55 V, deposition time: 15 s, equilibrium time: 10 s, voltage step: 0.005951 V, voltage step time: 0.6 s, sweep rate: 0.0099 V/s, pulse amplitude: 0.05 V, and pulse time: 0.04 s. The electrolytes used were 10 ml deionised water and 1 ml acetate buffer with pH of 4.64. The peak potential of ascorbic acid was found at 0.063 V. From five commercial roseile juice samples, three of them give a positive result on the ascorbic acid content. In the analysis of raw samples, it was found that the leaves have higher levels of vitamin C compared to calyces. As the conclusion, polarography technique was a successful applied for the determination of ascorbic acid in commercial roseile juice samples and raw samples such as roseile calyces and leaves.
| Item Type: | Monograph (Project Report) |
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| Uncontrolled Keywords: | Polarographic |
| Subjects: | R Medicine > R Medicine (General) R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research |
| Divisions: | Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Kesihatan (School of Health Sciences) > Monograph |
| Depositing User: | Mr Husnan Budin |
| Date Deposited: | 13 May 2024 06:27 |
| Last Modified: | 13 May 2024 06:27 |
| URI: | http://eprints.usm.my/id/eprint/60469 |
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