Abdul Lateef Khan, Khairunnisa
(2023)
Immunoproteomic Analysis And Serodiagnosis Of Salmonella Enteretica Typhi And Invasive Non-Typhoidal Salmonella Infections Using Differentially Extracted Whole Cell Bacterial Protein.
PhD thesis, Perpustakaan Hamzah Sendut.
Abstract
Typhoid fever is a potentially fatal infectious disease caused by Salmonella
Typhi. The disease is endemic in developing countries, but alarming number of cases
linked to domestic sources have been reported in industrialized countries. The clinical
presentation of typhoid fever is highly variable and often overlap with other acute
undifferentiated febrile illnesses (AUFI). Hence, accurate identification of isolates is
important for effective epidemiological intervention. The development of rapid, costeffective
diagnostic test is also crucial for early life saving treatment. Therefore,
comparative analysis was performed to characterize protein profiles of two
differentially extracted Salmonella serovars by sodium dodecyl sulphatepolyacrylamide
gel electrophoresis (SDS-PAGE). Separation of protein into three
different extractions revealed discriminative protein profiles between the two strains
which provided an effective adjunct that can be used as a tool for protein serotyping.
A standardized protocol of the dot Enzyme Immunoassay (dot EIA) with differentially
extracted antigens derived from S.Typhi and S.spp was established for definitive and
differential diagnosis of typhoid fever. The performance of the test was encouraging
with high sensitivity and specificity. Following the successful development of
'TYPHOIDYNE μSpot M-Ag Arrays, the antigenic components of the differentially
extracted proteins were further characterized by western blot (WB) assay.
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