Modifications Of Nk-92mi Cell Line Through Third Generation Lentiviral Vector

Chin, Ding Sheng (2023) Modifications Of Nk-92mi Cell Line Through Third Generation Lentiviral Vector. Masters thesis, Universiti Sains Malaysia.

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Abstract

In this study, NK-92MI cell line was transduced with RRL lentivirus carrying the genes for either intracellular GFP or surface CD16 receptor, with the addition of polybrene polycation. Results showed that GFP expression averaged at 3.49 % without polybrene and averaged at 7.18 % when polybrene was added. Extracellular CD16 expression averaged at 1.03 % without polybrene and increased to an average of 3.12 % with polybrene. This study demonstrated the feasibility of using the third generation RRL lentiviral vector for NK cell transduction.

Item Type:	Thesis (Masters)
Subjects:	R Medicine > R Medicine (General) > R5-130.5 General works
Divisions:	Institut Penyelidikan Perubatan Molekul (Institute for Research in Molecular Medicine INFORMM) > Thesis
Depositing User:	Mr Aizat Asmawi Abdul Rahim
Date Deposited:	14 Mar 2024 01:07
Last Modified:	14 Mar 2024 01:07
URI:	http://eprints.usm.my/id/eprint/60178

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