Mohd Fadzlah, Fadhlina Hazwani
(2023)
Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant.
PhD thesis, Perpustakaan Hamzah Sendut.
Abstract
Researchers have started to focus on vector management and implementation of
biological control to replace the hazardous effect of excessive insecticide usage. This study
was conducted to provide fundamental knowledge of bacteria diversity in breeding water and
midgut of Aedes albopictus. The identification of these bacteria is important for its roles as
paratransgenesis and oviposition attractant. The culture-dependent and culture-independent
methods were used for the comparative assessment of bacteria from artificial containers with
the presence and absence of larvae. Interestingly, the culture-dependant method showed a
higher bacterial diversity in breeding water with the presence of larvae but higher operational
taxonomic units (OTUs) in the absence of larvae by culture-independent method. These results
highlighted the importance of a type of bacterium that is responsible for the attraction or
deterrent effects towards mosquitoes. Higher microbiota diversity present in an adult
mosquito’s midgut compared to larval stage concluded that both environmental and vertical
inheritance are the microbiota sources in the midgut. The consistent presence of Bacillus cereus
and Proteus mirabilis in breeding water and both stages of Ae. albopictus mosquito’s midgut
served as potential early candidates for the paratransgenesis. The paratransgenic candidates
tested for B. cereus and P. mirabilis showed that the B. cereus was beneficial and more suitable
as paratransgenic candidate.
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