Molecular characterization of ornamental fosh (peocilidae) species by pcr-rflp of mitochondrial dna 12s rRNA gene

Azam, Mohamad Nor Fatihin Abshar Mohd Nor (2015) Molecular characterization of ornamental fosh (peocilidae) species by pcr-rflp of mitochondrial dna 12s rRNA gene. Project Report. Pusat Pengajian Kesihatan. (Submitted)

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Abstract

In this study four ornamental fish species from Peociliidae family namely Xiphophorus maculatus, Xiphophorus hellerii, Poecilia reticulate, Limia Vitatta and Poecilia shenops v/qtq used as a samples. Characterization of fish species was carried out by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) followed by digestion with endonucleases restriction enzymes. Three different enzymes were used such as Alu I, Dde I and Mse I. Based on result only Xiphophorus maculatus showed species specific profile after treated with Alu I enzyme. No species specific was seen in all fish species after treated with Mse I and Dde I.

Item Type: Monograph (Project Report)
Uncontrolled Keywords: Molecular characterization, ornamental fosh (peocilidae), mitochondrial dna
Subjects: R Medicine > RA Public aspects of medicine > RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine
R Medicine > RA Public aspects of medicine > RA440-440.87 Study and teaching. Research
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Kesihatan (School of Health Sciences) > Monograph
Depositing User: Mr Husnan Budin
Date Deposited: 08 Apr 2024 07:53
Last Modified: 08 Apr 2024 07:53
URI: http://eprints.usm.my/id/eprint/60011

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