Rahman, Nur Hasyimah Abdul
(2022)
Biomass Production Of Aureispira Sp. Ccb-qb1 And
Its Application As A Novel Bioflocculant.
Masters thesis, Universiti Sains Malaysia.
Abstract
Bioflocculants are important to remove pollutants, especially organic particles and
heavy metals from wastewater. Previous study showed that Aureispira sp. CCB-QB1
demonstrated cell aggregation when calcium ions are available. This suggested that these
bacterial cells are capable to adsorb the waste materials and have a great chance to be a
novel bioflocculant. Therefore, in this research, the methods for cultivation of Aureispira
sp. CCB-QB1 inside a bioreactor was studied. Then, the biosorption activity of the dead
cells toward heavy metals (Fe3+ and Cu2+) was tested. The QB1 dead cells were prepared
by treating the living cells using 0.5 % of formaldehyde. The dead cells were discovered
to show robust flocculating activity toward kaolin (initial concentration 0.2 %) in the
presence of 7 mM CaCl2. For cultivation inside flasks, 5 ml of the dead cell suspension
showed overall of 96 % of flocculating activity after shaken using both 150 and 200 rpm
agitation speeds. Besides, both tryptone and peptone were found to highly supported the
growth of QB1 cells. The presence of peptone in the cell culture also helps in the
aggregation of the QB1 cells. For cultivation inside a bioreactor, QB1 culture conditions
were optimized using Response Surface Methodology (RSM). The optimized conditions
were 3.39 % of sea salt concentration, 0.9 % of peptone concentration, and 29 h of
incubation period. Additionally
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