Mokhtar, Munirah
(2022)
Role Of Polysaccharides From Pleurotus Sajor-caju In The Immunomodulation Of Thp-1 Human Macrophage Cells.
Masters thesis, Universiti Sains Malaysia.
Abstract
Medicinal mushrooms have a diverse range of biological effects, which are excellent sources for pharmaceutical and nutraceutical use. They possess various valuable medicinal properties, including antitumor, hypocholesterolemic, anti-atherogenic, and anti-oxidative properties. Pleurotus sajor-caju is one of the Pleurotus spp. belonging to phylum Basidiomycota, well-known as oyster shaped mushroom (basidiocarps) and generally called oyster mushroom (OM). The present study was aimed to determine the ability of the water-soluble polysaccharides extract from Pleurotus sajor-caju extracts (PSC) in inducing the proliferation, immunostimulating and anti-inflammatory effects on THP-1 macrophage cells. First, the total carbohydrate and β-glucan contents were determined using the individual assay test. Next, the cell proliferation response was evaluated using the MTS assay. To understand the immunostimulating effect of PSC extract at the gene level, the expressions of selected cytokine genes in THP-1 cells were evaluated using real-time PCR. Nitric Oxide (NO) production and inducible nitric oxide synthase (iNOS) expression level were measured in the LPS-stimulated macrophages. The results obtained showed that 95% of total carbohydrate and 89% of β-glucan contents were detected in 50 mg PSC extracts, respectively. PSC extract was found to induce a marked increase in proliferative response on macrophage THP-1 cell in a dose-dependent manner, ranging from 10 μg/ml up to 80 μg/ml. At transcription level, PSC stimulated the expression of tumor necrosis factor-alpha (TNF-α), interleukin 1β (IL-1β), and interleukin 6 (IL-6) significantly (p<0.05) at various dosages of PSC extract.
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