Keng, Wong Kah (2019) 9th International conference on biosience, biochemistry & bioinformatics 2018. Project Report. Pusat Pengajian Perubatan. (Submitted)
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Abstract
Transient receptor potential cation channel subfamily M member 4 (TRPM4) is overexpressed in activated B-cell-like subtype of diffuse large B-cell lymphoma (ABC-DLBCL) associated with poor survival. In this study, its functions in the disease and the potency of its inhibitor 9-phenanthrol were investigated. The biological functions associated with TR.PM4 mRNA expression were examined through Gene Set Enrichment Analysis (GSEA) in ABC-DLBCL cases (n=15). The cytotoxicity of 9-phenanthrol in three ABC-DLBCL cell lines (SUDHL2, OCI-LY3, OCI-LYIO) was tested at six different concentrations (0.0InM, 0.1 nM, InM, lOnM, 25nM, 50nM). GSEA results showed that cell cycle gene sets conferred the highest number of gene sets representing 42% (n=21/50) of the top 50 most significantly enriched gene sets ranked according to false discovery rate (FDR; all 50 gene sets had FDRO.OI), followed by DNA replication (n=8/50; 16%) and RNA processing (n=8/50; 16%), suggesting the roles of TRPM4 in cell cycle progression and cellular division of ABC-DLBCL. In terms of the cytotoxicity effects of 9-phenanthrol, the resulting GI50 for all ABC-DLBCL cell lines ranged from 19nM-41,88nM. In conclusion, TRPM4 is potentially involved in the cell cycle progression and cellular division of ABC-DLBCL cells, and the TRPM4 inhibitor 9-phenanthrol was cytotoxic against ABC-DLBCL cells.
| Item Type: | Monograph (Project Report) |
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| Uncontrolled Keywords: | Cytotoxicity, diffuse large B-cell lymphoma, Gene Set Enrichment Analysis, TRPM4. |
| Subjects: | Q Science > Q Science (General) > Q1-390 Science (General) Q Science > QD Chemistry Q Science > QD Chemistry > QD415-436 Biochemistry R Medicine > R Medicine (General) > R5-920 Medicine (General) |
| Divisions: | Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Monograph |
| Depositing User: | Mr Husnan Budin |
| Date Deposited: | 27 Mar 2024 06:32 |
| Last Modified: | 27 Mar 2024 06:32 |
| URI: | http://eprints.usm.my/id/eprint/59762 |
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