Lim, Li Yang
(2022)
Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing.
PhD thesis, Perpustakaan Hamzah Sendut.
Abstract
Tapinoma indicum Forel (Hymenoptera: Formicidae) is a household pest that
is widely distributed in Asian countries. It is known as a nuisance pest that causes
annoyance and disturbance by constructing nests and foraging in building for food and
water. However, studies on T. indicum are limited, especially in the field of molecular
biology. Hence, this research aims to study the T. indicum population genetic structure
in Penang Island, Malaysia by using the novel microsatellite markers developed from
whole genome of T. indium. Objectives of this research include: to determine the T.
indicum population distribution in Penang island, Malaysia; to identify the
phylogenetic relationship of T. indicum based on the mitochondrial gene of
cytochrome c oxidase subunit Ⅰ (CO1) and 16S ribosomal DNA (16S rDNA); to design
novel microsatellite marker by screening microsatellite loci of T. indicum genome
sequenced from next-generation sequencing; to validate polymorphism of designed
microsatellite marker; to analyse genetic diversity and population genetic structure of
T. indicum using designed microsatellite markers. There was a total of 30 populations
collected from Balik Pulau, Gelugor and George Town. The CO1 sequences and 16S
rDNA sequences of T. indicum from each population were deposited and accessible
via Genbank (NCBI) database.
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