Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets

Rajasegar, Arulvilee (2022) Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets. PhD thesis, Perpustakaan Hamzah Sendut.

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As a monopodial plant, Phalaenopsis is traditionally propagated by off-shoot cutting or division. However, this resulted in a low multiplication rate and hinders the growth of the mother plant and therefore inefficient for large-scale production. Micropropagation has provided a valuable alternative method for the large-scale production of identical orchid plantlets within a short period. The study aimed to improve the proliferation of protocorm-like bodies (PLBs) of Phalaenopsis Fuller’s Light × Phalaenopsis Purple Heart through the micropropagation method. The thin cell layer culture (TCL) approach generated a 2.16 times higher neo-PLB formation rate than the conventional half-PLB explants. The medium composition of full MS medium supplemented with 3% sucrose, 2% banana extract, and 15% coconutwater significantly produced the highest PLB fresh weight (2.499 g) after 4 weeks of culture. The Phalaenopsis PLBs further developed into shoot and root using 1.0 mg/L BAP and NAA mg/L. The incorporation of 1.5 mg/L of glutamine resulted shoot formation with 70% while 1.5 mg/L of arginine increased root formation by 87%, wherein the cultures were differentiated into complete plantlets.

Item Type: Thesis (PhD)
Subjects: Q Science > QH Natural history > QH1-278.5 Natural history (General)
Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User: Mr Hasmizar Mansor
Date Deposited: 13 Dec 2023 01:28
Last Modified: 13 Dec 2023 01:28

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