Rajasegar, Arulvilee
(2022)
Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets.
PhD thesis, Perpustakaan Hamzah Sendut.
Abstract
As a monopodial plant, Phalaenopsis is traditionally propagated by off-shoot
cutting or division. However, this resulted in a low multiplication rate and hinders
the growth of the mother plant and therefore inefficient for large-scale production.
Micropropagation has provided a valuable alternative method for the large-scale
production of identical orchid plantlets within a short period. The study aimed to
improve the proliferation of protocorm-like bodies (PLBs) of Phalaenopsis
Fuller’s Light × Phalaenopsis Purple Heart through the micropropagation method.
The thin cell layer culture (TCL) approach generated a 2.16 times higher neo-PLB
formation rate than the conventional half-PLB explants. The medium composition
of full MS medium supplemented with 3% sucrose, 2% banana extract, and 15%
coconutwater significantly produced the highest PLB fresh weight (2.499 g) after
4 weeks of culture. The Phalaenopsis PLBs further developed into shoot and root
using 1.0 mg/L BAP and NAA mg/L. The incorporation of 1.5 mg/L of glutamine
resulted shoot formation with 70% while 1.5 mg/L of arginine increased root
formation by 87%, wherein the cultures were differentiated into complete
plantlets.
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