Mokhtar, Khairaniidah
(2012)
Identification of differentially expressed
gene/s observed in stem cells derived
extracted human tooth.
Project Report.
Universiti Sains Malaysia.
(Submitted)
Abstract
The aim of this study was to identify the differentially expressed genes (DEGs) in SHED and DPSCs by GeneFishing™ DEG method using the arbitrary primer pairs provided. Two bands
which were highly expressed in SHED and three bands in DPSCs were isolated purified and
sent for sequencing. Sequencing analysis revealed these to be TIMP Metallopeptidase
Inhibitor 1 (TIMP1}, (A09), and ribosomal protein s8, (RPS8), (A16) in SHED and collagen,
type I, alpha 1, (COL1AP), (A20), follistatin-like 1 (FSTL1), (A17), lectin, galactosidebinding,
soluble, 1, (LGALSP), (A16) in DPSCs. TIMP1 is involved in degradation of the
extracellular matrix, cell proliferation and anti-apoptotic function and RPS8 is involved as a
rate-limiting factor in translational regulation; COL1A1 is involved in the resistance and I elasticity of the tissues; FSTL1 is an autoantigen associated with rheumatoid arthritis;
LGALS1 is involved in cell growth, differentiation, adhesion, RNA processing, apoptosis, and
malignant transformation. The gene expression patterns of SHED and DPSCs might be useful
in determining the detailed functional roles of the relevant genes applicable to stem cell
therapies, which paves way to be used as I multipotent cell sources for genetic and tissue engineering technology.
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