Pei, Cheung Tian
(2012)
Genotyping of angiotensin-1 converting enzyme
insertion/deletion gene polymorphism in
malay ischemic stroke patients.
Project Report.
Universiti Sains Malaysia.
(Submitted)
Abstract
The human angiotensin-1 converting enzyme (ACE) gene contains an insertion or
deletion (I/D) of Alu repeat element in intron 16 of the chromosome 17 (17q23). This
polymorphism has been widely investigated to determine its effects in causing ischemic
stroke but inconsistent results are obtained. Thus, this study aims to genotype the ACE
I/D polymorphism to determine its genotypic frequency among Malay ischemic stroke patients. The study subjects comprised of 27 Malay ischemic stroke patients that were recruited from HUSM. ACE I/D gene polymorphism was determined by genotyping the
SNP rs4341 polymorphism using PCR-RFLP method. The frequencies of ACE
polymorphism (II, ID and DD genotypes) were determined and presented in the form of
percentage. The results showed that ACE II genotype was present in all Malay ischemic
stroke patients (100%) while ID and DD genotypes did not make any contribution to the
genotypic frequency. Therefore, the ACE II genotype is responsible for the occurrence
of ischemic stroke among Malay stroke patients. However, the result is a preliminary
finding in which larger and extensive studies can be performed based on it.
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