Khaw, Shin Yuan
(2022)
Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells.
Masters thesis, Perpustakaan Hamzah Sendut.
Abstract
Astaxanthin is a carotenoid with multiple health benefits including antioxidant
and anticancer properties. An astaxanthin-hyperproducing X. dendrorhous mutant was
generated due to the growing preference for natural pigment. This work aimed to study
the astaxanthin-rich X. dendrorhous extracts and its effect on MCF-7 and MDA-MB-
231 cells. Spectrometric, TLC and HPLC analysis evidenced that mutant M34
produced astaxanthin in higher quantity and purity than the wild type. Mutant
astaxanthin extract showed a higher scavenging activity compared to wild type
astaxanthin extract in DPPH assay. MTT assays proved that wild type and M34
astaxanthin extracts were not toxic towards the non-cancer origin MCF-10A cells.
Wild type and mutant astaxanthin extracts exhibit growth-inhibitory effect in dosedependent
manner in MCF-7 and MDA-MB-231 cells. Mutant astaxanthin extract
showed a lower IC50 than wild type extract and was more effective in inhibiting both
MCF-7 and MDA-MB-231 cells. The IC50 values of wild type and mutant astaxanthin
extracts did not exceed the required upper limit of 30 μg/ml.
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