Masri, Mohamad Fadzirul Hadzli Mohamad
(2009)
A comparative in-vitro study of cultured
primary normal human epidermal
kera tinocyte on artificial dermal
regeneration template and fibrin scaffold.
Project Report.
Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia.
(Submitted)
Abstract
Skin plays an important role in regulating our body homeostasis. Once the dermal
layer is destroyed, it cannot heal by itself, a transplant or artificial dermal regeneration
template is needed in order to restore its function. This study aims to explore the
successfulness in the cultivation of primary normal human epidermal keratinocyte
(pNHEK) within INTEGRA® dermal regeneration template {INTEGRA ®-DRT) and
fibrin. Keratinocytes were seeded on INTEGRA ®-DRT and fibrin and observed on three
day basis until one week. During the culture, cells were supplied with medium containing
elevated calcium in order to promote cell differentiation. The cell viability analysis,
growth pattern and three dimensional views were conducted using Laser Scanning
Confocal Microscope (LSCM). From the analysis, pNHEK were grown on both scaffolds
and the proliferation rate was better in fibrin compared to INTEGRA ®-DRT (p < 0.05).
However, the penetration rates are better in INTEGRA ®-DRT scaffold compared to
fibrin. In conclusion, both scaffolds are suitable to be developed as skin substitute
depends on bum situation.
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