Mohamed, Mohaini
(2009)
Isolation of adipose stem cells from human skin
adipose tissue.
Project Report.
Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia.
(Submitted)
Abstract
Traditionally, stem cells are isolated from bone marrow. These cells have the potency to
develop into various types of cells. Thus, mesenchymal stem cells from bone marrow
can be used in treating the diseases that need cells therapies or regenerative of cells.
Much of the procedures that involve the aspiration of stem cells from bone marrow were
invasive and painful. Thus, many researches try to find the alternative source of stem
cells. Adipose tissue which is ubiquitous in human body and can be easily isolated
promise as a novel source for tissue engineering and regenerative medicine. When
compared with bone marrow stem cells, adipose-derived stem cells may develop into
adipocytes, chondrocytes, osteocytes and myocytes in vitro. In this research project,
adipose tissues from humans were collected and isolated in vitro. The isolation
procedures involved centrifugation, filtration and culturing with DMEM: F-12 (1:1), FBS
and PenStrep. Adipose stem cells were isolated from human subcutaneous adipose
tissue, and appeared as fibroblast-like morphology observed under inverted phasecontrast
microscope.
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