Khairuddin, Ili Zawani
(2015)
Multi target analysis of HeLa cells
radiosensitivity.
Project Report.
Universiti Sains Malaysia.
(Submitted)
Abstract
The main objective of this study is to investigate the radiosensitivity of
HeLa cells based on analysis using multi target model.
Methods: The cell irradiations were performed using photon beam of energy 6 MV, 10
MV and electron beam of energy 6 MeV, 15 MeV. Samples were setup in full scatter
condition using solid water phantom and bolus. Each sample was exposed to doses
range from 1 to 10 Gy. Clonogenic assay were performed to obtain the cell survival
curve data and the data were later analysis using Multi Target (MT) model. Analysis
using Linear Quadratic (LQ) was also done for comparison. From the result, it shows that the survival curve for electron
was steeper compared to photon beam. Therefore, HeLa cells were more radiosensitive
to electron beam compared to photon beam. The MT model shows dominant reason of
death cell due to multiple events cell killing which represents by Do. The value for Do
for 6 MV, 10 MV, 6 MeV and 15 MeV were 2.42385, 3.39997, 2.42385, and
4022.75834 respectively. The MT model was found to be fitted better compare to LQ
model for different energies and especially for high dose. HeLa cell is more radiosensitive toward photon beam than electron beam
for MT Model while for the LQ model, cells were more radiosensitive to electron beam
compare to photon beam. The Multi Target model describe the cell survive curve for
high dose better than Linear Quadratic model.
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