Kit, Tan Tiong
(2013)
Immunomodulatory properties of tualang honey
on BALB/c mice.
Project Report.
Universiti Sains Malaysia.
(Submitted)
Abstract
Local natural product extracts has been gaining great focus from research and
therapeutic aspect. Similarly, the outcome of studies on one of nation’s renowned
honey Tualang (TH) (Koompassia excelsa) has profoundly anchored its board
aptitude in anti-inflammatory, anti-oxidant and anti-microbial properties. This
research focuses on the study of the immunomodulatory properties of TH in
BALB/c. TH was orally administered daily for 14 days to BALB/c male mice
(5/group) in dose ranging from 0.5 g/kg, 1.5 g/kg and 3.0 g/kg namely Low group,
Medium group and High group. Pre-treatment and post-treatment body weights were
measured. Upon sacrificing, the spleen was weighed and then homogenized. The
splenocytes were stained with various surface markers antibodies namely
CD3+/CD4+(T helper), CD3+/CD8+(T cytotoxic), CD14+ (macrophage) and CD19+
(B lymphocyte) and immune cell populations were obtained by using flow
cytometer. On the other hand, proliferation assay of splenocytes were done by using
CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS). Body weight
in Control, Medium and High groups showed increment after the treatment but body
weight of mice in Low group decreased upon completion of TH treatment. BALB/c
mice treated with TH showed increased populations of CD3+/CD4+, CD3+/CD8+,
CD14+ and CD19+ compared to control group. Proliferation analysis of splenocytes
as well as spleen weight obtained from TH-treated mice also showed an increment.
The results revealed the immunostimulant potential of TH in mice by enhancing
lymphocyte populations especially T helper (CD3+/CD4+), cytotoxic (CD3+/CD8+)
and B (CD19+) cells.
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