Yen, Pong Sze
(2013)
Pro inflammatory responses of thp-1 macrophage cell line
infected with recombinant bcg (rBCG) expressing the MSP-1C of
Plasmodium falciparum.
Project Report.
Universiti Sains Malaysia.
(Submitted)
Abstract
Phagocytosis of macrophage is an important innate defence mechanism against
malaria parasites. In the presence of parasites, the macrophage gains its proinflammatory
responses through the production of pro-inflammatory cytokines and
nitric oxide (NO) which allow it to eliminate the parasites from infected red blood
cells. In this study, the ability of human macrophage cell line, THP-1 infected with a
recombinant BCG (rBCG) clone expressing the 19-kDa C-terminus of the merozoite
surface protein-1 (MSP-1C) of Plasmodium falciparum in stimulating proinflammatory
responses indicating by phagocytosis activity, the production of IL-12,
NO and inducible nitric oxide synthase (iNOS) were determined. The results
demonstrated that the phagoctic activity and expression of NO were higher in cells
infected with rBCG compared to cells infected with BCG, while no significant
differece was observed on the levels of IL-12 production and iNOS protein
expression by these cells. Thus, this study shown that the rBCG vaccine might had
the potential to be used as innate immune activator for malaria infection. However,
further investigations must be carried out to prove the hypothesis.
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Phagocytosis of macrophage is an
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