Ting, Ong Jin
(2013)
Anticandidal Activity of Quercus infectoria “Nut Gall” Extracts on Candida spp.
Project Report.
Universiti Sains Malaysia.
(Submitted)
Abstract
Anticandidal activity of Quercus infectoria “nut gall” organic and aqueous extracts
were studied on Candida spp. The Quercus infectoria nut gall extracts were obtained
through successive extraction with different solvents in increasing order of polarity
firstly with petroleum ether, followed by chloroform, ethanol, and finally water. Five
ATCC strains of pathogenic Candida species namely Candida albicans, Candida
glabrata, Candida tropicalis, Candida krusei and Candida parasilopsis were used in
this study. For preliminary anticandidal activity testing, an agar dilution method was
employed by using diluted extracts with the concentration of 0.4 mg/ml, 0.8 mg/ml,
1.6 mg/ml and 3.2 mg/ml respectively. The determination of minimum inhibitory
concentration of the extracts against Candida spp. based on the macroscopic
observation of growth colony after 48 hours of incubation on SDA. Our study found
that all tested Candida species were susceptible to amphotericin B (< 1 pg). Besides,
all extracts did not show inhibition at concentration below 1.6 mg/ml, only C.
albicans and C. parapsilosis were inhibited by chloroform extract while other
Candida species did not show growth inhibition to all other extracts at concentration
of 1.6 mg/ml and above. The chloroform extract of Q. infectoria showed better
anticandidal activity than the other organic and aqueous extracts.
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