Siddiqua, Snigdha Niher Tabassum
(2023)
Bacterial leakage and marginal
adaptation of three bioceramics pulp
dressing material.
Masters thesis, Universiti Sains Malaysia.
Abstract
This study aims to evaluate the sealing ability and marginal adaptation of three
different bioceramics pulp dressing materials using a bacterial leakage test and
scanning electron microscope (SEM). Fifty-five recently extracted lower first
premolars were randomly divided into five groups, with three experimental groups
(n=15), a positive control group (n=5) and a negative control group (n=5). The samples
were instrumented with a modified coronal pulpotomy procedure except for the
negative control group (n=5). Different types of bioceramics dressing material were
placed in the cavity 3mm thickness in group 1 (Biodentine), group 2 (MTA) and group
3 (ProRoot MTA). There was no dressing material placed in the positive control group.
Samples were placed in an incubator at 37℃, 100% humidity, for 24 hours to allow
the material to be set, after the placement of the composite restoration. Two layers of
nail varnish were applied, and the 3mm root tip was removed. The bacterial leakage
test was performed using Enterococcus faecalis, and one sample from each
experimental group was sliced and examined under SEM for marginal adaptation. Data
analysis was conducted under the One-way ANOVA test, completed by Tukey’s post
hoc test. The groups observed a significant difference in sealing ability and marginal
adaptation (p<0.05), wherein Biodentine showed bacterial leakage on day 6 (7%), and
on day 14 (80%), MTA showed on day 9 (14%) and on day 14 (40%), ProRoot showed
on day 11 (7%) and on day 14 (33%). From SEM, ProRoot MTA demonstrated the
least gap between the dressing material and significantly higher penetration in dentinal
tubules. ProRoot MTA demonstrated better sealing ability and marginal adaptation
compared to other groups. The finding indicates that ProRoot MTA would be the best
pulp dressing material for the clinical setting.
Keywords: Bacterial leakage test, bioceramics, Biodentine, MTA, ProRoot
MTA, Pulpotomy, SEM.
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