Almazaraa, Khadeeja Ameen Khudhair
(2021)
Comparative analysis of amoebic
seropositivity among Kelantan
cattle farm dwellers using CSA- and rCL-IgG-ELISA.
Masters thesis, Universiti Sains Malaysia.
Abstract
Kelantan cattle farm dwellers are one of the high-risk groups for invasive amoebiasis,
as the farming activities involve the use of untreated water source such as underground
and well water. Screening for invasive amoebiasis by crude soluble antigen (CSA) and
recombinant c-terminal lectin (rCL) antigen provide different interpretation on the
disease exposure. Assay based on the former antigen has higher affinity towards
variety of anti-amoebic antibodies, while the latter is specific for invasive amoebiasis.
The present study aimed to study the amoebic seropositivity among Kelantan cattle
farm dwellers using customised CSA- and rCL-IgG-ELISA. The two different
antigens were produced and used for the screening of the serum samples based on
previously optimised parameters. Thirty blood donor serum samples were used for the
determination of cut-off values for the two customised assays. RIDASCREEN®
Entamoeba histolytica IgG was used as the reference test. From the analysis, the
reference test detected 31 (34.83%) positive cases, while CSA- and rCL-IgG ELISA
detected 41 (46.07%) and 17 (19.10%) cases, respectively. The two customised assays
showed moderate agreement with the commercial test, nonetheless, CSA-ELISA
showed higher agreement with the findings of commercial test, as compared to rCLELISA.
From ROC curve analysis, the area under the curve of CSA-IgG-ELISA was
higher than that of rCL-IgG-ELISA, as well. In conclusion, CSA- and rCL-IgG-ELISA showed different seropositivity; findings by the rCL-IgG-ELISA are lower and could
be more specific for invasive amoebiasis.
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