Abdullah, Nurul Asma
(2016)
In vivo teratogenecity and anti-allergic of
lignosus rhinoceros in an asthmatic robert model
and their possible bioactive constituents.
Project Report.
Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia.
(Submitted)
Abstract
Lignosus rhinoceros (L. rhinoceros) or locally known as Tiger's Milk mushroom, is traditionally used in
the treatment of various diseases including asthma by indigenous communities in Malaysia. This study
investigated volatile constituents of L. rhinocerus using gas chromatography mass spectrometry (GC-MS),
teratogenic and male fertility, immunomodulatory properties and anti-asthmatic effects of L. rhinocerus in
animal models. Sequential extractions of L. rhinoceros using five solvents demonstrated 44 constituents were
identified. Compounds from the fatty acid group were the most predominant (68.58%) and the main
constituent was linoleic acid (49.39%). This study also demonstrated that oral administration of L. rhinocerus
did not noticeably show treatment-related signs of toxicity in both male and female rats. We also suggested
that oral administration of L. rhinocerus extract did not result in remarkable teratogenicity in rats. The antiasthmatic
potential of L. rhinoceros extract was further investigated on ovalbumin (OVA)-induced airway
inflammation Sprague Dawley rats. Treatments with L. rhinocerus extract significantly ameliorated related
asthmatic parameters in the induced rats. Specifically, L. rhinocerus extract comparatively reduced
eosinophils numbers in BALF, lgE in serum, Th2 cytokines (IL-4, IL-5 and IL-13) levels in BALF, eosinophil
infiltrations in the lungs as well as modulate regulatory T cell numbers in the airways. lmmunomodulatory
effects of L. rhinocerus extract demonstrated improved splenocyte population mainly of co3•co4• and
Co3•cos• as well as improved splenocytes proliferation and phagocytosis activity and controlled nitric oxide
(NO) production. In conclusion, L. rhinocerus has the potential to be used as an alternative for the treatment
of allergic asthma.
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