Hanan, Rosnani Che AWang @ Abdul
(2014)
The evaluation of loop mediated isothermal
amplification (LAMP) for the detection of porphyromonas
gingivalis using heat-treated saliva.
Project Report.
Universiti Sains Malaysia.
(Submitted)
Abstract
Porphyromonas gingivalis has been implicated as a major pathogen of periodontitis.
Thus far, there have been no reported studies on attempted to detect P. gingivalis by
Loop Mediated Isothermal Amplification (LAMP) using heat-treated saliva. Therefore,
this study aims to evaluate the applicability of LAMP assay for detection of P.
gingivalis in heat-treated saliva samples. DNA of saliva samples from periodontitis
patients and healthy subjects were prepared by using heat-treated method and
commercial kits. The results obtained were then compared with those omit obtained by
conventional PCR assay. In periodontitis patients, P. gingivalis was detected in 4/5
(80%) extracted DNA using commercial kit in both LAMP and PCR assays. Two out of
five (40%) of heat-treated saliva of periodontis patients were positive by LAMP and none was detected by PCR assay. In healthy subjects, no sample was positive by PCR in both heat-treated saliva and extracted DNA using commercial kit. However, 2/5 (40%) of heat-treated saliva and 1/5 (20%) of extracted DNA using commercial kit were positive by LAMP assay. These findings suggested that LAMP assay was corresponded
to those of conventional PCR when using extracted DNA prepared from commercial kit.
However, LAMP assay using heat-treated saliva was more sensitive than the
conventional PCR, suggesting the potential of LAMP assay using heat-treated saliva
samples in detection of P. gingivalis.
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