Mohamad, Dasmawati
(2017)
Evaluation of Glass Ionomer Cements
(GICS) properties with coumarin derivatives as
antibacterial agent.
Project Report.
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia.
(Submitted)
Abstract
Glass Ionomer Cements (GIC) are widely used as dental restorative materials due to their aesthetics
features and fluoride content. However, a capability of fluoride content in GIC to inhibit bacteria growth in
an oral environment was insufficient for a long term which may leads to secondary caries. Therefore, the
objective of this research was to fabricate a new GICs with coumarin derivatives as an antibacterial agent.
:3-Acetylcoumarin (1) and hydrazine thiosemicarbazide coumarin derivatives (2) have been successfully
5ynthesized at laboratory scale. Their structural identification was confirmed by Fourier Transform Infrared
(FTIR) and Nuclear Magnetic Resonance (NMR) analysis. Both synthesized compounds were fabricated
with GICs at percentage of 0.5, 1.0, 1.5, 2.0 and 2.5. The antibacterial activities of tested materials were
assessed by agar diffusion test (ADT) and bacteria growth. The effect of incorporation of 3-acetylcoumarir
(3-AC) on the mechanical properties and surface morphology of glass ionomer cement (GIC) were
evaluated. Flexural strength of the specimens were analysed using Shimadzu AGX-Pius while
morphological evaluation of the specimens were observed using Scanning Electron Microscope. An ill·
vitro cytotoxicity study of the synthetised coumarin derivatives were screened using a 3-(4,5·
dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MIT) assay on Human Periodontal Ligament
Fibroblast (HPDLF) cells. Release profile of 3-Acetylcoumarin (GIC-1) and hydrazinyl thiosemicarbazide
of coumarin derivatives (GIC-2) at three different concentrations of 0.5, 1.0 and 1.5 % were evaluated
using Microplate Reader up to 30 days. Both synthesized coumarin derivatives showed very promising
properties as an antibacterial agent to be incorporated with GIC.
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