Amira Raudhah, Abdullah
(2013)
An in-vitro study on the effect of carica papaya crude aqueous
extract on the voltage-gated sodium channel (VGSC)
mediated motility on mda-mb 231 breast cancer cell line.
Other.
Pusat Pengajian Sains Perubatan.
(Submitted)
Abstract
Voltage-gated sodium channel (VGSC) in the line of suitable target markers for cancer
therapy has been extensively studied by researchers over recent years. Variety of study
provides suggestive evidence on the involvement of VGSC in regulating growth and
expansion of cancer cell together with its role in metastasis. The predominant and
highly expressed VGSC in breast cancer cells is the neonatal splice variant (nNavl.5).
We aimed to target the specific VGSC markers, nNavl .5 gene expression towards
controlling the rate of metastasis in breast cancer. Carica papaya is our choice
candidate in down regulating the gene expression of nNav 1.5 due to its local anesthetic
properties as well as well-known role in exhibiting the anti-cancer or anti-proliferative
properties. MTI assay and Trypan Blue assay procedure were conducted to test the
toxicity and viability of MDA-MB-231 breast cancer cell in response to C. papaya
aqueous crude extract treatment at 0.1 μg/mL, 1.0 μg/mL and 10.0 μg/mL. Moreover,
we performed the lateral motility assay and PCR in order to determine the effects of C.
papaya treatment at similar concentration on MDA-MB-231 cells motility and nNavl.5
gene.
Results and data analysis provide suggestive evidence that at the concentrations tested,
the aqueous crude extract of C. papaya was non-toxic and also did not affect the growth
of MDA-MB 231 cells. Interestingly, whilst the extract was none effective on the cell's
growth, lateral motility of MDA-MB-231 cells was reduced, suggesting that the effect
of the extract on reducing motility was indisputable. Consequently, the expression of
nNavl.5 gene was down-regulated as the concentration of C. papaya treatment
increased, suggesting the mode of action of the extract on reducing motility might
involve VGSC nNavl.5. However the data analysis appears to be insignificant for both.
Therefore, in this thesis, we provide suggestion for future study to obtain a better result
and prove the ability of C. papaya in controlling breast cancer metastasis.
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