Mohd Khairi, Md Daud
(2013)
A preliminary investigation on the
biocompatibility studies of cellulose
from oil palm residue as surgical
biomaterial.
Other.
Pusat Pengajian Sains Perubatan.
(Submitted)
Abstract
Cellulose is the world's most abundant natural, renewable, and biodegradable
polymer. An in vitro biocompatibility test was carried out to look at cytotoxicity
characteristic of our locally produce cellulose phosphate on osteoblast and
chondroblast cell lines. Cellulose samples were prepared from the oil palm residues.
The cell line used in this study was human osteoblast and Chondroblast obtained
from the American Type Culture Collection. An in vitro cytotoxicity test was carried
out on the cellulose material using extraction/indirect methods. For human
osteoblast, the concentration for IC-50 was determined at 60mg/ml while no IC-50
was observed for human chondroblast cells. The results suggest the biocompatibility
of cellulose as biomaterial on human cell lines is good and not cytotoxic. Cellulose is the world's most abundant natural, renewable, and biodegradable
polymer. Polysaccharides, like cellulose, are the polymer group with the longest
and widest medical applications experience because of their unique properties:
nontoxicity, water solubility or high swelling ability by simple chemical modification,
stability to temperature and pH variations, and a broad variety of chemical
structures 1
. The biocompatibility of some cellulosics is well documented. Oxidized
cellulose is used as a wound dressing2
. Regenerated cellulose hydrogels
(cellulose regenerated by the viscose process-CRV) have been investigated as
implantable materials in orthopaedic surgery. Their good matching with
mechanical properties of bone, as well as their biocompatibility has been
I
demonstrated3
Chemically modification via phosphorylation further stimulates
bone induction4. Research innovation on the use of cellulose and its derivatives in
medical application was conceived and developed all over the country but thus far
not in Malaysia.
In vitro cell culture methods are the basic starting point whereby biological
responses to materials are determined initially, as required by a number of
standardization agencies5. These methods have the advantages of simplicity,
good sensitivity and reproducibility and are widely used in the initial evaluation of
biocompatibility of materials. In this study, an in vitro biocompatibility test was
carried out to look at cytotoxicity characteristic of our locally produce cellulose
phosphate on osteoblast and chondroblast cell lines.
ific
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