Md. Azman PKM Seeni, Mohamed
(2009)
Eight Annual AACR International Conference on Frontiers in
Cancer Prevention Research
Houston, Texas, U.S.A.
06-09 Disember 2009.
Other.
Pusat Pengajian Sains Perubatan.
(Submitted)
Abstract
Eptdemrologrcal studres have demonstrated a posrtNe cooeldtion between
consumption of vE'(jeldbles, frurts and bevt:r ages with reduced mk of canre. It 1s
esumated there are around 8,100 plant speer~ 'in the Mal.~ysian rain forests.
wrth 1 0% of them reported to have some medicinal value. HOWE'\Ier, to date m
Malaysta, not much investigdtion has been donP on chE>mopreventiw actrvitrP.S
on cancer although Malaysran plants are an exclusrve !.Ottrce of effe<trve
themopreventrve agents and therefore. thrs batkground leads to the premrse
that our local plants such as Streblus asper could have greater potentral for the
chemoprevention a<tJVrlles. Streblus asper rs well known as an expensrve bon~11
plant whrch rs rndrgenous to tropccal countnes such as Malaysia. Thailand. Srr
Lanka and lndra It rs used tradiuonally rn leprosy. pries. drarrhea. dysentery.
elephantrasis and cancer tt finds place in AyurvediC Pharmacopoeia of India and
also been descrrbed m some monographs, but none have reported rts activity as
chelllOpfeventive ~ts and the underlyrng me<hanisms remarn undt>ar In the
present study, we try ro identify its brologrcal properues and the cytotoKrdty
efle<t on normallrver and kidney cells. Us1ng the osteosarcoma cells as our 1n
vrtlo model, the ICSO of Streblus asper root eXtract was deterrnrned and
observed rts efle<t on the osteosarcoma cells morphology that changes w1thrn
trme. the anu prolifer atrve pattern and live-death analysis under confocal
mrcroscope analysrs. The results showed thdt the root extracts drd not contarned
any heavy metals compound such as mercury and cadmium and With less
arsenrc (0 02 ppm) clnd plumbum (0.07 ppm) and ~ing non·<)'totoxrc effect
on vero cells (normal krdney cells} and WRL·68 (E'tls (normal liver cells) Our
HPLC profiling analysrs also r!'Vealed antroxidants compounds exrst rn the
Streblus asper root extracts such as caffeic acid which has been shown to dCI as
a carcrnogenrc rnhibrtOI Although the low·fevef of antr-oxrdants been found
from the extracts but rt strll can rnhrbrt thl' growth of the osteosarcoma cells
wtuch also exerl dpoptosis features l1ke cell sh11nkage (atrophy) and
vocalizatron rn trme and dose-dependent manner The plant extracts IC50 doses
was Jt 0.05% of root extracts and rt also demonstrated the ant•·prolifLoratrve
effe<t by suppressed the cells growth as early as 12 hours of treatment and
marked cell death t1il day 6 On live'ileath analys~s under confocal mrcroscope
usrng Calcern and Ethrdrum starnrng confrrmed that Streblus asper root extract
exer1 cell death to osteosarsoma cells. This study IS Just a prelimrnary study as to
identrfy rts pharmucologrcal propertres on c,:~~crnogenesrs and further
rnvestrga110n rs strll on-gorng to develop rt as the chemopreventrve agent
espec:ialty to determrne the srgnaling pathway involved
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