Fong, Pooi Har
(2020)
Paretage Assigment Gene
Expressio Of Growth Related Gene In
Snakehead Murrel, Channa Striata.
PhD thesis, Perpustakaan Hamzah Sendut.
Abstract
Haruan (Channa striata) is a fresh water fish known for its medicinal value
and is an important source of protein in Southeast Asia. The demand for this fish is
still high; hence this species has good market potential for aquaculture industry in
Malaysia. Furthermore, snakehead farming is not popular and there is yet a selective
breeding as well as systematic hatchery programmes developed in Malaysia. Hence,
the first objective of this study was to investigate the feasibility of using the existing
microsatellite markers in genotyping C. striata for parentage identification and
pedigree management. The next objective is to establish a reference for
transcriptome library for gene expression study. With the transcriptome library,
differentially expressed genes in response of fast and slow growth could be identified
and applied in snakehead murrel selective breeding programmes. Accurate parentage
identification for pedigree management is integral to a successful systematic
hatchery programme especially in aquaculture industry to prevent inbreeding
depression. Hence, the feasibility of seven pairs of microsatellite markers
characterized previously for a population genetics study of C. striata was evaluated
in this study. A total of 211 offspring from 22 brood stocks kept in six separated
tanks were genotyped according to tanks and were later assigned back to these 22
brood stocks using five different parentage assignment software. Among these
software, Colony was the best software to successfully allocate the offspring back to
their parents. In addition, the results of the parentage allocations revealed that mating
behaviour of this species is polygamous and polyandry.
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