Ghani, Balqis Ab.
(2007)
Expression Of PhaC1 And PhaC2 From
Pseudomonas Sp. USM4-55 In
Escherichia Coli Expression System.
Masters thesis, Universiti Sains Malaysia.
Abstract
Polyhydroxyalkanoates (PHAs) are synthesized intracelullarly by a variety of microorganisms as reserve materials for carbon and energy source.
PHA synthase (PhaC) is the key enzyme in the polymerization of PHAs.
Pseudomonas sp. USM4-55 is a local isolate which is able to produce a blend
of short chain length and medium chain length of polymer. Genes coding for
PhaC (phaC1 and phaC2) from Pseudomonas sp. USM4-55 were amplified
(each with a size of 1.7 kb) from the genomic DNA, cloned into pGEMR-T for
sequencing and then expressed. For expression studies, pQE-30 was used as
the expression vector under the control of PT5 promoter. These clones were
named pQE-C1 and pQE-C2, and were induced with 0.01 mM of isopropyl-p-Dthiogalactoside
(IPTG) at ODeoo of 0.6.
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