Shafie, Anis Syahirah Mohd
(2022)
The role of tualang honey in
modulating nociceptive responses in
the thalamus of rapid eye movement
(REM) sleep deprivation rat model.
Masters thesis, Universiti Sains Malaysia.
Abstract
Rapid eye movement (REM) sleep deprivation has been postulated to contribute
to the alteration of nociceptive responses; however, the causes and effects are poorly
understood. The aim of this study was to investigate the effects of REM sleep
deprivation on nociceptive behaviour score, histological changes, the level of oxidative
stress parameters, and the level of NMDA R2 receptors in the thalamus of REM sleepdeprived
rat model. Furthermore, this study also determined the effects of Tualang
honey on the parameters investigated. Forty-eight Sprague-Dawley male rats were
equally divided into four groups (n=12); free moving control (FMC), REM sleep
deprivation for 72 hours (REMsd), REM sleep deprivation for 72 hours pretreated with
Tualang honey (REMsdH) and tank control (TC). Tualang honey (1.2 g/kg body weight/
day) was given by oral gavage for 30 days prior to the REM sleep deprivation
procedure. Following the experimental period, formalin test and nociceptive behaviour
were conducted. The rats were sacrificed, and the thalamus was removed for histological
examination and quantification of oxidative stress parameters and NMDA receptors
levels. REMsdH group showed a significant decrease in nociceptive behaviour score
compared to REMsd group (p<0.05). The number of Nissl-stained neurons was
significantly higher in REMsdH compared to REMsd (p<0.05). Dark neurons were
observed in the VPL region of the REMsd group but not in the REMsdH group.
REMsdH showed a significant increase (p<0.001) in GSH, GR, SOD, and CAT levels but a significant decrease (p<0.001) in MDA and NMDA R2 levels in the thalamus
compared to REMsd. In conclusion, the administration of Tualang honey has protective
effects against the adverse effects of REM sleep deprivation.
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